Mouse Monoclonal Antibodies Targeting SARS-CoV-2 Nucleocapsid Protein
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At A Glance
Researchers at Colorado State University have developed several mouse monoclonal antibodies targeting the SARS-CoV-2 nucleocapsid protein.
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Coronavirus nucleocapsid protein plays a key role in coating the viral RNA genome during virion formation, and overall pathogenesis. Additionally, the nucleocapsid protein has been used as a diagnostic marker for active coronavirus infection. There are very few antibody reagents currently available that are specific for SARS-CoV-2 nucleocapsid, as most monoclonal antibodies that are commercially available were generated against SARS-CoV nucleocapsid protein and are cross-reactive for SARS-CoV-2 nucleocapsid.
- Clones were screened by ELISA for reactivity towards recombinant SARS-CoV-2 nucleocapsid protein, counter screened against bacterial lysates and heterologous 6xHis-tagged proteins
- Top 18 clones were tested for efficacy in Western blot analysis with recombinant SARS-CoV-2 nucleoprotein as well as SARS-CoV-2 infected cell lysate
- Clones are currently being tested for activity in immunofluorescene and immunohistochemistry, and sandwich ELISA assays.
- Antibodies specifically target SARS-CoV-2 nucleocapsid
- Nucleocapsid protein is a diagnostic marker for active coronavirus infection
- Application in ELISA assays, reducing/denatured western blots, immunofluorescence assays, sandwich ELISAs, gene sequences, and others
Last updated: June 2020