Radioimmunometric Assay to measure Anti-SARS-COV2 Antibodies in Serum
Collaboration and Funding Interest Welcome
Torrance “Terry” M Nett
At A Glance
Researchers at Colorado State University have developed a radioimmunometric assay (RIA) to detect and quantitate antibodies against SARS-COV2 in serum.
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- Assay is an offshoot of the classic double antibody radioimmunoassay
- Directly measure the amount of radiolabeled pathogen-specific antigen that is bound in immune complexes following the addition of a serum sample
- Not only can detect the presence of pathogen-specific antibodies, but can quantitate the concentration in serum
- Developing detection using assay with sputum (to avoid invasive blood draws)
- Assay will be validated under guidance of the FDA (EUA)
- High sensitivity – see Figure 1 below
Five samples were analyzed at a dilution of 1:10. The % binding vs the log PRNT90 (antibody titer) were graphed, showing a correlation between the PRNT90 and % binding of r = 0.98
- More sensitive than other types of assays
- Can quantify antibody concentration
- Cost is significantly less than ELISA assays
- Clinical Labs with RIA capabilities
- Pharma and University Research
Last updated: June 2020
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#CSUInvents – #TechTuesday! Researchers at Colorado State University have developed a radioimmunometric #assay (RIA) to detect and quantitate #antibodies against #sarscov2 in #serum. Not only can the assay detect the presence of #pathogen-specific antibodies, but it can quantitate the concentration in #serum. Currently, researchers are developing detection using the assay with sputum (to avoid invasive blood draws). Advantages include high sensitivity and significantly lower cost than #ELISA-based assays.
Inventors include: Torrance M Nett, Professor of #biomedicalscience CSU College of Veterinary Medicine and Biomedical Sciences and Eric Weber, Ph.D., co-founder and Director of Operations, Endolytics, LLC
#immunoassay #serology #serological #AUTM #FLC #covid19research #CSU #technologytransfer #innovation