Translation and Tethering (TnT) Plasmid

For Live Cell Imaging

Unaltered Image by ZEISS Microscopy from Germany

Opportunity

Available for Purchase at Kerafast –
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Inventors

Timothy Stasevich
Charlotte Cialek

At A Glance

Researchers at Colorado State University have developed fluorescence microscopy tools to image proteins in living cells that are difficult if not impossible to see using standard techniques. They are using these techniques to answer long-standing questions about how epigenetic factors contribute to gene misregulation in human disease. Currently they are focusing on two hard-to-see protein populations: (1) nascent proteins in the process of being translated (while still attached to RNA) and (2) post-translationally modified proteins, particularly modified chromatin and modified transcription machinery.

The “Translation and Tethering” TnT biosensor is a combination of the nascent chain tracking translation reporter and mRNA tethering reporters. The TnT biosensor is adaptable to any factor since tethering/recruitment is through a tethering cassette and not the endogenous RNA binding domain of a specific factor. TnT can be used to screen effects such as translation as well as up/down regulation of proteins. Additionally, TnT can be used to study translation factors as well as effects of subcellular localization on translation by tethering TnT mRNA to a specific location.

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Licensing Director

Steve Foster
Steve.Foster@colostate.edu
970-491-7100

Reference No.:  2021-087

Translation and Tethering (TnT) Plasmid
The TnT plasmid is a nucleic acid-based biosensor to test the specific impacts of translation control factors by microscopy.

Highlights:

  • Combination of the nascent chain tracking translation reporter and mRNA tethering reporters
  • Adaptable to any factor since tethering/recruitment is through a tethering cassette
  • Useful for screening translation as well as up/down regulation of proteins
  • Study translation factors as well as effects of subcellular localization

Last updated: August 2021

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